The Gastrointestinal Microbial Assay Plus (GI-MAP) was designed to assess a patient’s microbiome from a single stool sample, with particular attention to microbes that may be disturbing normal microbial balance and may contribute to perturbations in the gastrointestinal (GI) flora or illness. The panel is a comprehensive collection of microbial targets as well as immune and digestive markers. It screens for pathogenic bacteria, commensal bacteria, opportunistic pathogens, fungi, viruses, and parasites. It primarily uses multiplex, automated, DNA analysis to give integrative and functional medicine practitioners a better view into the gastrointestinal microbiome.
Methodology
Quantitative polymerase chain reaction (qPCR)
Indications
 Autoimmune molecular mimicry (if caused by pathogens)
Diarrhea
Digestion
Foodborne illness
Gastritis
Gastroenteritis (if it has viral causes)
Gut dysbiosis
Immunity
Inflammation
Suspected bacterial, parasitic or viral pathogenesis (incl. detection of antibiotic resistant bacteria)
Analytes 
BACTERIAL PATHOGENS
Campylobacter
C. difficile Toxin A
C. difficile Toxin B
Enterohemorrhagic E. coli
E. coli O157
Enteroinvasive E. coli/Shigella
Enterotoxigenic E. coli LT/ST
Shiga-like Toxin E. coli stx1
Shiga-like Toxin E. coli stx2
Salmonella
Vibro cholerae
Yersinia enterocolitica
H. pylori
Virulence Factor, babA
Virulence Factor, cagA
Virulence Factor, dupA
Virulence Factor, iceA
Virulence Factor, oipA
Virulence Factor, vacA
Virulence Factor, virB
Virulence Factor, virD
PARASITIC PATHOGENS
Cryptosporidium
Entamoeba histolytica
Giardia
VIRAL PATHOGENS
Adrenovirus 40/41
Norovirus GI
Norovirus GII
NORMAL/COMMENSAL BACTERIA
Akkermansia Mucinophilia
Bacteroides fragilis
Bifidobacterium spp.
Clostridia (class)
Enterobacter spp.
Enterococcus spp.
Escherichia spp.
Faecalbacterium prausnitzii
Lactobacillus spp.
BACTERIAL PHYLA
Bacteroidetes
Firmicutes
Firmicutes/Bacteroidetes Ratio
ADDITIONAL DYSBIOTIC/OVERGROWTH BACTERIA
Bacillus spp.
Enterococcus faecalis
Enterococus faecium
Morganella spp.
Pseudomonas spp.
Pseudomonas aeruginosa
Staphylococcus spp.
Staphylococcus aeureus
Streptococcus spp.
Methanobacteriaceae (family)
POTENTIAL AUTOIMMUNE TRIGGERS
Citrobacter spp.
Citrobacter freundii
Fusobacterium spp.
Klebsiella spp.
Klebsiella pneumoniae
Mycobacterium avium subsp. paratuberculosis
Prevotella spp
Proteus spp.
Proteus mirabilis
FUNGI/YEAST
Candida albicans
Candida spp.
Geotricum spp.
Microsporidia spp.
Rhodoturula spp.
OPPORTUNISTIC VIRUSES
CMV- Cytomegalovirus
EBV- Epstein Bar Virus
PARASITES & WORMS:
PROTOZOA
Blastocystis hominis
Chilomastix mesnelli
Cyclospora spp.
Dientamoeba fragilis
Endolimax nana
Entamoeba coli
Pentatrichomonas hominis
WORMS
Ancyclostroma duodenale
Ascaris lumbricoides
Necator americanis
Trichuris trichiura
Taenia spp
INTESTINAL HEALTH MARKERS:
DIGESTION
Elastase-1
Steatocrit
IMMUNE RESPONSE
SIgA
Anti-gliadin SIgA
INFLAMMATION
Calprotectin
GI MARKERS
ß-Glucuronidase
Occult Blood - FIT
ANTIBIOTIC RESISTANCE GENES (Optional add-on):
Phenotypes | HELICOBACTER
Amoxicillen
Clarithromycin
Fluroquinolines
Tetracycline
Specimen
Stool sample 
Container
1x orange-capped stool vial
Patient preparation
Please refrain from taking aspirin for two days prior to collecting your sample.
Never discontinue prescription medication without first consulting your physician.